How to Improve Your Skincare Routine with Peptides in 2025
Peptides are quickly gaining popularity among skincare enthusiasts, and it’s no surprise why. Known for their ability to rejuvenate the skin, improve firmness, and enhance overall skin health, peptides are a game-changer in modern skincare routines.
In this guide, we’ll explore everything you need to know about peptides—from their benefits and potential downsides to the different types, how they work with other skincare ingredients, and tips for incorporating them into your routine.
Ready to take your skincare game to the next level? Let’s dive in and make 2025 your best year yet for radiant, healthy skin.
What Are Peptides?
Peptides are chains of amino acids. They help manage and improve skin texture, especially firmness, elasticity and smoothness. Peptides are effective because they are small and can easily be absorbed into the body to signal these functions.
Benefits of Peptides for the Skin
The benefits of peptides for the skin include the following:
- Slow aging and reduced wrinkles and fine lines.
- Skin tightening through collagen and elastin stimulation
- Sun damage protection and alleviation through pigmentation management
- Anti-inflammation
- Strengthened the skin barrier
- Wound healing by elevating healthy tissue production
- Improved skin hydration through hyaluronic acid promotion and moisture retention
- Antioxidant properties to reduce oxidative stress and free radical damage
- Balances the skin texture and tone.
Downsides of Peptides
Since they are naturally occurring agents, adverse reactions to peptides are rare. However, you can experience sensitivity, rashes and itching from topical creams.
Types of Peptides
The types of peptides you can use in skincare include carrier peptides, signal peptides, enzyme inhibitor peptides, and structural peptides. Below are some details about each one of them:
Carrier Peptides
Carrier peptides deliver needed proteins to the body directly. They boost the production of collagen by delivering trace minerals to the skin, and include peptides like Copper peptide (GHK-Cu).
Signal Peptides
Owing to their name, these peptides send signals to the skin to stimulate the production of elastin, collagen and other proteins relevant to skin health. Examples of signal peptides are:
- Palmitoyl Pentapeptide-4
- Palmitoyl Tripeptide-1
- Palmitoyl Tripeptide-5
- Acetyl Tetrapeptide-17
- Epidermal Growth Factor (EGF)
- Fibroblast Growth Factor (FGF)
Enzyme Inhibitor Peptides
Enzyme inhibitor peptides inhibit the production of enzymes that break down naturally occurring collagen in the body, thus elevating the levels of collagen in the body. Peptides in this class include Palmitoyl Tripeptide-5, which in addition to being a signal peptide, inhibits enzymes (like MMPs) that degrade collagen.
Structural Peptides
These make up key structural components in or are modified forms of relevant peptides. They are delivered to the skin directly to improve the production of relevant peptides. Examples include Hydrolyzed Collagen and Elastin.
10 Peptide Ingredients for Skincare
Below, we review 10 peptides ingredients you should consider including in your skincare routine:
Copper Peptide GHK-Cu
As a carrier peptide, Copper Peptide GHK-Cu delivers trace elements like copper to the body and as a signal peptide, it helps boost the body’s ability to produce collagen.
Copper peptides are naturally occurring in human plasma, urine and saliva but are produced at a lower rate with age. So, they may come in handy for older people. Copper peptides are particularly great at promoting wound healing and reducing oxidative damage in the skin.
When to Use Copper Peptide GHK-Cu
- For Anti-Aging: Copper peptides are highly effective for boosting collagen and elastin, making them a great choice if you're dealing with wrinkles, fine lines, or sagging skin.
- To Heal Skin: They are excellent for repairing damaged skin, speeding up wound healing, and reducing scars.
- For Skin Firming: Copper peptides help tighten and firm the skin by increasing the production of proteins in the skin.
- After Microneedling: Since copper peptides promote skin healing, they can be applied after microneedling or other skin treatments to boost recovery.
- For Reducing Inflammation: Copper peptides have anti-inflammatory properties, which make them ideal for soothing irritated or sensitive skin.
- In Your Nighttime Routine: Copper peptides can be used as part of your evening skincare routine to allow for better absorption and repair overnight. They are best used at night when your skin isn’t exposed to sunlight or environmental stressors.
When Not to Use Copper Peptides
- Metal Allergies: If you are sensitive to metal, you might experience irritation or allergic reactions to copper peptides.
- During Active Breakouts: While copper peptides can help with wound healing, they can aggravate active acne in some cases. It’s better to address active breakouts with other ingredients like salicylic acid first.
Palmitoyl Pentapeptide-4
Also known as Matrixyl, Palmitoyl Pentapeptide-4 is a potent stimulator of collagen production, making it one of the most effective peptides for reducing fine lines and wrinkles.
Palmitoyl Pentapeptide-4 can be used in both your morning and evening routines without irritation. For best results, apply after cleansing and toning, before any heavy moisturizers or oils.
When to Use Palmitoyl Pentapeptide-4
- For Anti-Aging.
- Under Makeup: Since it's non-irritating and lightweight, it's safe to use under makeup.
Palmitoyl Tripeptide-1
Also known as Matrixyl-3000 (when combined with Palmitoyl Tetrapeptide-7), Palmitoyl Tripeptide-1 helps boost the production of type-1 collagen and is suitable for all skin types. Its standout feature is its ability to repair damaged skin and improve skin thickness.
When to Use Palmitoyl Tripeptide-1
- For Skin Repair: It helps repair damaged skin, so it can be used if you're dealing with sun damage, post-acne marks, or other signs of skin aging.
- With Hydrating Ingredients: This peptide works well alongside hyaluronic acid and ceramides, enhancing skin hydration and moisture retention.
- In Combination with Other Peptides: Palmitoyl Tripeptide-1 often works synergistically with peptides like Palmitoyl Tetrapeptide-7 to improve skin firmness and reduce inflammation.
- For Daily Use: It’s gentle enough for morning and evening applications. You can use it as part of a daily skincare routine to enhance collagen production over time.
Palmitoyl Tripeptide-5
Palmitoyl Tripeptide-5 is a synthetic peptide, which doubles as an enzyme inhibitor and signal peptide. It inhibits enzymes like MMPs responsible for the degradation of collagen and as a signal peptide, it signals the creation of collagen. Also, it can enter the dermis and epidermis and signal the growth of collagen.
When to Use Palmitoyl Tripeptide-5
Palmitoyl Tripeptides is a better choice for people who want the anti aging effects of retinol but are sensitive to it. It is particularly effective in higher concentrations when in moisturizers, creams, and serums. However, it is still important to find the right balance for your skin by consulting an expert.
How to Apply Palmitoyl Tripeptide-5
- Cleanse the affected area first and dry off.
- If using for the first time, do a patch test to prevent a possible allergic reaction.
- Apply the product, following the application instructions.
- Apply moisturizer if the initial product isn’t one but ensure that the earlier product has been absorbed into the skin.
- Then finally apply your sunscreen.
All in all, gradually incorporate Palmitoyl Tripeptide-5 into your skincare routine.
Acetyl Tetrapeptide-17
Acetyl tetrapeptide is sometimes known as Palmitoyl Tetrapeptide-7 or "Pal-GHK" in some formulations. It is used in creams, serums and other skincare products as an anti-wrinkling, anti-sagging agent, and so on. Acetyl Tetrapeptide is particularly effective for skin firmness as it strengthens the dermal structure.
When to Use Acetyl Tetrapeptide-17
- After Environmental Stress: If your skin is affected by UV exposure or pollution, Acetyl Tetrapeptide-17 helps by reducing oxidative stress and reinforcing the skin barrier.
Epidermal Growth Factor (EGF)
Epidermal Growth Factor (EGF) helps to restore the body on a cellular level. It stimulates fibroblasts and epithelial cells responsible for the production of collagen and elastin. It also speeds up the wound healing from sun damage and hyperpigmentation.
EGF can be an excellent replacement for retinol as it prevents skin thinning commonly associated with retinol.
When to Use Epidermal Growth Factor (EGF)
- For Skin Repair and Regeneration: EGF is ideal for accelerating skin healing and improving cell turnover. It’s particularly beneficial after treatments like microneedling, chemical peels, or laser therapy, helping the skin recover faster.
- To Reduce Scarring: It’s effective in reducing the appearance of scars, including acne scars, due to its ability to regenerate new, healthy skin cells.
- For Improving Skin Texture: It can smooth out rough or uneven skin texture, giving your complexion a more youthful and refined appearance.
- Daily Use: EGF is gentle enough for daily application, and regular use can result in long-term improvements in skin health and appearance.
When Not to Use Epidermal Growth Factor (EGF)
- In an Active Cancer: EGF stimulates cell growth, so it’s generally advised to avoid this peptide when at risk of or diagnosed with cancer, as it may stimulate the growth of unhealthy cells.
- In Unstable Formulations: EGF is sensitive and may become ineffective in products with harsh preservatives, alcohol, or unstable formulations. It's best to use products specifically designed to preserve the integrity of EGF.
Fibroblast Growth Factor (FGF)
FGF is another growth factor linked to collagen and elastin production. It stimulates the growth and function of fibroblast cells.
By stimulating fibroblast cells, which are responsible for the production of collagen, elastin, and glycosaminoglycans, FGF enhances skin structure by promoting the production of connective tissue, making the skin firmer, more elastic, and youthful. Unlike other peptides, it focuses more on rejuvenating the skin's support structure.
When to Use Fibroblast Growth Factor (FGF)
- For Skin Rejuvenation: FGF is excellent for stimulating fibroblasts, the cells responsible for producing collagen, elastin, and hyaluronic acid. This helps rejuvenate the skin’s structure, leading to a more youthful appearance.
When Not to Use Fibroblast Growth Factor (FGF)
- If You Have Active Skin Conditions: It’s best not to use FGF on inflamed or acne-prone skin as it stimulates cell growth, which might aggravate conditions like active acne or rosacea.
- If You're at High Risk for Cancer: Like EGF, FGF promotes cell growth, so it’s not recommended for individuals with cancer concerns, as it could potentially accelerate the growth of unhealthy cells.
Hydrolyzed Collagen
Collagen is a protein that is vital to the skin and for skincare in general; it is responsible for skin firmness and elasticity. However, hydrolyzed collagen is collagen that has been broken down to ensure easy absorption into the body.
Hydrolyzed collagen helps to hydrate the skin, making it a popular ingredient in anti-ageing and moisturizing products. By boosting skin elasticity and reducing dryness. It's especially beneficial for maintaining moisture and improving the overall texture of the skin, and you’ll get the best anti-aging results if you use it for at least eight weeks.
Supplements that consist of hydrolysed collagen can be in the form of powders, capsules, liquids or tablets, with fish-based supplements being the best source. That said, powdered hydrolyzed collagen supplements are popularly used for wound dressing.
While getting collagen from supplements is more efficient than getting it from food, food sources of collagen should not be ignored.
When to Use Hydrolyzed Collagen
- For Hydration and Moisture: Hydrolyzed collagen is highly effective at improving skin hydration. Use it if your skin feels dry, dehydrated, or lacking in moisture.
- To Improve Skin Elasticity: It helps boost skin elasticity, making it beneficial for mature skin or anyone looking to maintain firmness.
- For Smoother Skin Texture: Hydrolyzed collagen can improve skin texture, reducing the appearance of roughness or dryness, resulting in a more even and supple complexion.
- Daily Use: You can apply hydrolyzed collagen daily in the morning or at night. It’s suitable for all skin types, especially those needing extra hydration or anti-ageing support.
- Post-Treatment Care: It’s helpful after skin treatments like chemical peels or exfoliation, as it aids in maintaining moisture balance and skin recovery.
When Not to Use Hydrolyzed Collagen
- If You Have Allergies: Hydrolyzed collagen is often derived from animal sources (such as fish or bovine). If you have a known allergy to these sources, avoid using them or opt for plant-based collagen alternatives.
There have been no records of severe reactions to HC, but there may also be mild gastrointestinal effects like nausea and heartburn.
Elastin
Elastin is responsible for the skin’s elasticity (expansion and contraction) contributing to a firm and youthful appearance. It works alongside collagen to maintain the skin’s structure and prevent sagging.
When to Use Elastin
- For Improving Skin Elasticity: Elastin is key for enhancing skin elasticity, helping to maintain the skin's ability to stretch and return to its original shape. It's beneficial for addressing sagging or loss of firmness.
- To Support Skin Structure: Use elastin if you're looking to strengthen the skin's support structure, as it contributes to the skin's resilience and flexibility.
- Daily Application: Elastin can be used in morning or evening routines and is suitable for all skin types, particularly those experiencing loss of firmness or elasticity.
Melanotan
Melanotan stimulates the production of melanin leading to increased pigmentation and a tanned appearance without the need for sun exposure. As such, it offers protection against UV damage.
There are two types of Melanotan: Melanotan I (MT-I) and Melanotan 2 (MT-II) and both stimulate and imitate the production of melanin leading to increased pigmentation in the skin.
MT-I has little to no side effects, but it is scarce and more expensive. However, MT-II can cause side effects such as acne, nausea, and dizziness.
When to Use Melanotan
- For Tanning: Melanotan is used to stimulate melanin production, leading to a natural-looking tan without sun exposure. It's ideal for those looking to achieve a tanned appearance safely.
- To Enhance Pigmentation: Use Melanotan to increase skin pigmentation, which can be beneficial for individuals with conditions like vitiligo or those seeking to even out their skin tone.
- In Controlled Dosages: When using Melanotan, it's crucial to follow recommended dosages and usage guidelines to achieve desired tanning effects while minimizing potential side effects.
- With Sun Protection: Combine Melanotan with sunscreen to ensure protection from UV damage and to maintain skin health.
When Not to Use Melanotan
- Without Medical Supervision: Avoid using Melanotan without medical guidance. Incorrect usage or dosages can lead to unwanted side effects or health risks.
- If You Have Skin Conditions: People with certain skin conditions, such as melanoma or other skin cancers, should avoid using Melanotan due to potential risks associated with increased melanin production.
- In Combination with High UV Exposure: While Melanotan enhances pigmentation, it does not replace the need for sun protection. Excessive UV exposure can still damage the skin, so it's important to use sunscreen.
- During Pregnancy or Breastfeeding: There is limited research on the safety of Melanotan during pregnancy or breastfeeding, so it's best to avoid it during these times unless advised by a healthcare provider.
Can Peptides Be Used With Vitamin C?
Some experts believe that you should not use peptides with vitamin C, as they may be less effective together, especially if the peptide is copper-based. Copper-based peptides can oxidize the ascorbic acid in vitamin C, reducing its effectiveness.
That said, some experts believe that peptides can be used with vitamin C without a significant drop in their individual effectiveness. To err on the side of caution, you can use one for your daytime routine and the other for nighttime routine.
What Can I Use With Peptides?
You can use peptides with hyaluronic acid, niacinamide, and ceramides.
What Not To Use With Peptides?
You should not use peptides with the acid family AHAs (alpha-hydroxy acid) and BHAs (beta-hydroxy acid), high levels of retinoids, damaged skin, and formulations with harsh preservatives.
How to Improve Skincare With Peptides?
To improve your skincare routine with peptides, follow these steps:
- Choose the Right Peptides for Your Skin Type: For aging and wrinkles, look for peptides like Palmitoyl Pentapeptide-4 (Matrixyl), Copper Peptides, or Acetyl Hexapeptide-8 (Argireline). For hydration, Palmitoyl Tripeptide-1 and Palmitoyl Tetrapeptide-7 are excellent for improving moisture retention.
- Layer Peptides Properly: Apply peptide products (serums or creams) after cleansing and toning but before heavier moisturizers or oils. This ensures the peptides penetrate the skin effectively. Peptides work well with hydrating ingredients like hyaluronic acid and non-acidic antioxidants.
- Combine Peptides with Complementary Ingredients: Peptides pair well with niacinamide to help boost collagen production and improve skin texture. Avoid combining peptides with strong acids (e.g., AHAs or BHAs) or retinol, as these may reduce peptide effectiveness.
- Be Consistent: Use peptide-infused products daily to see long-term benefits. Results typically appear after 4–6 weeks of consistent use.
- Use Sunscreen: Always apply sunscreen during the day, especially when using anti-ageing peptides, as sun exposure can degrade collagen and hinder their effects.
- Choose Peptide-Infused Eye Creams: For under-eye wrinkles and puffiness, use eye creams containing peptides specifically designed for this delicate area.
Final Thoughts
Peptides play a vital role in modern skincare, offering impressive benefits like skin tightening, enhanced collagen production, and improved overall skin texture. While peptides offer significant anti-aging and healing advantages, it is essential to use them correctly and pair them with compatible ingredients for optimal results.